Medical Applications of Ozone: Part 2

4th International Symposia on Ozone Applications
April 6th through 9th 2004, Havana City, Cuba
Abstracts

Ozone therapy in ophthalmology:

Application of ozone therapy and electromagnetic field in primary open angle galucoma.
Ferrer L., Góngora, Santos D., Pérez and Menéndez S. (Cuba)

Primary open angle glaucoma is a chronic illness, producing serious visual problems if it is not diagnosed appropriately and treated correctly. It represents a big health problem, that affects economically the family and the state, besides being presented in ages where man has complete physical and intellectual capacities. It constitute the second cause of blindness and visual disability in the world. Increase intraocular pressure is a factor of high risk in this disease, although exist patients with normal intraocular pressure with loss of their visual function, after topical or surgical treatments, with a high deterioration of the optic nerve. Since 1989, we are carrying out a rehabilitation program for these patients suffering of glaucoma, using ozone therapy and electromagnetic field, together with hypotensive treatment. Ozone therapy is applied by rectal administration (ozone concentration: 40 mg/L and 200 mL) in cycles of treatment of 20 sessions, twice per year. In 1993, this rehabilitation program was generalized to the whole country, being applied in those hospitals where exist the ozone therapy department and the electromagnetic equipment. The results of these 15 years demonstrate a positive result in the visual function (visual field and visual acuity) with more than 70 % of improvement. According to the visual evoked potentials, a decrease in the latency of the P 100 wave was achieved. Also, a decrease in the intraocular pressure (more than 50 %) was achieved. No side effects were observed.

Ozone therapy in patients with retinitis pigmentosa.
Copello M., Menéndez, S., Erguía F. and Menéndez N. (Cuba)

Retinitis pigmentosa (RP) is characterized by progressive night blindness. The aim of this study is to determine the efficacy of ozone therapy in patients with RP. A controlled, randomized, double blind clinical trial involving 68 patients was performed. Patients were divided into 2 groups: ozone, patients were treated with ozone by rectal administration (dose = 10 mg), during 15 sessions; control, as ozone group but using oxygen. The main outcome variable was the visual field area (VFA), measured in the Goldman Perimetry (with V4 and white stimulus). To all patients VFA was measured at the beginning of the study and every month during one year follow-up. Significant improvement (SI) was only assessed if an improvement of 25 % of basal VFA was presented and maintained in 2 consecutive monthly exams. Results demonstrated a SI in 88.2 % of patients treated with ozone in comparison with 23.5 % achieved in the control group (p = 0.003). In the ozone group, SI was more frequent and of high magnitude in the first month of follow-up (76.5 % of patients, with 42.9 % of mean improvement). A significant difference in the main VFA during the first semester comparing with the basal VFA was achieved (Z = 27), but not occurring the same in the second semester (Z = 1.13). In the first semester 78.4 % of patients improved their visual field, while in the second semester only 20.8 %. VFA tend to stabilize beyond a mean time of 6.83 months with a loose in SI afterward. A temporal positive effect of ozone therapy, over the natural course of RP, was found. It could be useful to apply ozone therapy in the first stages of the disease and at six-month intervals in order to enhance visual capabilities in RP patients.

Ozone therapy in retinitis pigmentosa.
Pérez L.J. (Cuba)

The aim of this study is to determine the efficacy of ozone therapy for patients with retinitis pigmentosa. A double blind, placebo controlled study was designed for three years, in which 186 patients, with ages from 15 to 65 years participated, suffering from typical forms of retinitis pigmentosa. After signing an informed consent, a complete general and ophthalmologic examination was performed, before and after the treatment and 6 months later. Snellen visual acuity was measured (less than 2.5 lines considered non significant changes) and Goldmann kinetic visual field with 31.5 asb. background and V-4e target. The sample was divided into 2 groups: ozone - 165 patients treated daily, with ozone by major autohemotherapy, during 15 sessions, using an ozone concentration of 40 mg/L and a volume of 100 mL, in 100 mL of blood; control - 21 patients, 100 mL of their blood were extracted, collected in a flask with anticoagulant and then immediately reinfused. The results demonstrated, 6 months after the treatment, that 21 % of patients treated with ozone maintained their visual acuity improvement, the 74.2% remained the same and 4.8 % worsen. According to visual field area, 46.7 % of patients maintained their improvement, 41.9 % remained the same and 11.35 % worsen. Significant differences were observed between both groups, with lower frequencies of improvements in control group and larger proportions of worsening. Conclusions: Ozone therapy is a useful treatment for patients with RP, although its action is temporary. Repeated administration after 6 months is recommendable to maintain its action.

Ozone therapy and redox balance in patients with Retinitis Pigmentosa.
Menéndez S., Hernández F. and Copello M. (Cuba)

Ozone therapy is capable to stimulate the endogenous antioxidant defense system, by means of an oxidative preconditioning, preparing the host to face physiopathological conditions mediated by reactive oxygen species. The aim of this paper is to correlate the oxidative stress or redox index of patients with Retinitis Pigmentosa with the clinical efficacy of the ozone therapy treatment. Twenty seven patients were treated with a cycle of ozone therapy (20 sessions) every 6 months. Ozone was applied by rectal insufflation at a dose of 8 mg (ozone concentration: 40 mg/L and a volume of 200 mL). Nineteen patients received 2 cycles of ozone therapy and 8 patients received 4 cycles of ozone therapy. For the oxidative stress diagnosis several biochemical parameters were measured: superoxide dismutase, catalase, glutathione peroxidase, glutathione S transferase, reduced glutathione, conjugate dienes, thiobarbituric acid reactive substances, total hydroperoxides and redox index. The clinical evolution was measured by means of visual field and visual acuity. An improvement of the visual field and the visual acuity of 70 and 19 % of patients, respectively, were achieved. Visual acuity diminished in 1 patient (3 %) and remained the same in 78 %. Respect to the redox index, patients maintained its figure in the range of no stress, at the end of the treatment, in spite of the oxidative stress present in patients with Retinitis Pigmentosa. The oxidative stress diagnosis is a valuable system in order to personalize the dose of ozone treatment. Ozone therapy is a good therapeutic option for patients with Retinitis Pigmentosa, without side effects.

Ozone therapy in patients with diabetic retinopathy.
Hernández R., Ramírez T., Guerrero M., Betancourt J., Rubio L. and Izquierdo N. (Cuba)

The aim of this paper is to evaluate the efficacy of ozone therapy in the treatment of Non-Proliferative Diabetic Retinopathy (NPDR), an ocular complication of diabetes. Twenty five patients were examined through clinic biometry, in order to confirm the absence of other complications of diabetes. The inclusion criteria were: patients between 40 and 60 years old, males and females who suffered loss of visual acuity produced by retinal changes due to diabetic retinopathy. Patients were treated with ozone, by rectal insufflation, at concentration between 35-40 mg/L and 200 mL, one daily, for 15 sessions. This cycle of treatment was repeated every six months, up to 2 years. An ophthalmologic evaluation (visual acuity, direct and indirect ophthalmoscopy, retinophoto and fluorescein angiography) of the patients was performed at the beginning and at the end of every cycle of ozone therapy. In 72 % of the patients a visual improvement was achieved, with a slow disappearance of microaneurysm, intraretinal hemorrhages, hard exudates, microhemorrhages and retinal edema. From the second cycle on, this improvement was maintained in direct relation with a clinic biometry within the normal parameters (cholesterol, glycemia and creatinine), mainly on patients who suffered from Diabetes type II. No visual improvement was observed in the rest of the patients (28 %) during 2 years of follow-up. An appropriate metabolic control of patients who suffer from mild NPDR was achieved, allowing a favorable recovering of their vision, as well as an improvement in their quality of life. On the basis of the stimulant effect of medical ozone in the processes of oxygen metabolization and blood circulation we can recommend this therapy for the treatment of patients suffering of Non-Proliferative Diabetic Retinopathy.

Ozone therapy in senile macular degeneration.
Soto G., Falcón J., Montesino I. and Soler L. (Cuba)

Taking into account the incidence of senile macular degeneration (SMD) and low efficacy of conventional treatment, the aim of this study is to evaluate the efficacy of ozone therapy in patients with SMD of dry type. A retrospective study was performed in 22 patients assisted at the Ophthalmologic Service of the Medical Surgical Research Center (CIMEQ). Patients were diagnosed through a complete ophthalmologic examination and fluorescent angiography. Ozone was applied by major autohemotherapy (at an ozone concentration of 40 mg/L and 100 mL) and by rectal application (at an ozone concentration of 40 mg/L and 200 mL), during 20 sessions. A high percent of patients (54.5 %) treated were between 6l and 70 years. White race predominate (45.4 %), as well as female sex (68.2 %). The results demonstrated an improvement (with an average of 2 tenths-visions) of their visual acuity in 80 % of the patients treated with ozone. Ozone therapy could be a good therapeutic choise for patients suffering of SMD of dry type.

Ozone therapy in orthopedy:

Knee and ankle arthrosis treated with ozone therapy.
Portela A. (Spain)

The aim of this work is to demonstrate the efficacy of intra-articular ozone therapy in different patients suffering of knee and ankle pathologies. The results and discussion of cases are presented in videos. It was used ozone by intra-articular administration, at a concentration between 20-30 mg/L and a volume between 7-10 mL, 3 times per week, during 1 or 2 months. Three patients suffering from knee arthrosis (patient of 65 years old suffering of genu varum, young patient with condylar degeneration and a patient with knee infection) and 3 suffering from ankle arthrosis (patient with peroneal tibialis astragalus arthrosis, young sportman patient with malleolar lesion and adult female with ankle arthrosis) were treated. Good results were achieved, demonstrated by X-ray and clinical evidences.

Intradiscal ozone therapy for the treatment of disk herniation.
Pérez A. and Menéndez S. (Cuba)

In many painful diseases and especially in the presence of inflammatory processes, ozone therapy has been used with very good results. Spinal diseases are the most common cause of disability in persons under the age of 45. The aim of this paper is to evaluate the efficacy of intradiscal ozone therapy in the treatment of lumbar and cervical disk hernia. We studied 45 patients (of both sex), with 6 months follow-up at least, 30 suffering of lumbar hernia (L5 - S1, 16 patients and L4 - L5, 14 patients) and 15 with cervical hernia (C6 - C7, 9 patients and C5 - C6, 6 patients). All of them were preliminarily submitted to clinical examination, electromyography, CT and MRI. After local anesthesia, we injected the disk, with 18 - 20 G needles (15 cm of length), under fluoroscope guidance, with 10 mL (cervical hernia) or 15 mL (lumbar hernia) of a mixture of oxygen and ozone at a concentration of 20 - 25 mg/L. The treatment was repeated two to ten times, once per week, in dependence of the pain elimination. At the end of the treatment, physical exam, neurologic and orthopedic tests were performed. CT follow-ups were carried out 4 months later. Signs and symptoms of disk herniation are caused by compression of either nerve roots or the spinal cord. The specific signs and symptoms are based on which part is compressed and at which level the neural structures are compressed. The total resolution of the symptoms was achieved in 88 % of patients. An attenuation of the symptoms, without interference with the patient daily activities, were obtained in the rest of the patients (12 %) treated. Patients with no benefits, 0 %. No patients underwent surgery after the treatment. Cervical hernia needed less ozone therapy sessions to eliminate the pain (between 2 - 4 sessions), however lumbar hernia needed between 6 and 10 sessions to obtain the same results. A great advantage of this method is that practically has no contraindications and it does not require hospitalization. Ozone therapy has shown to be a promising therapy for patients with herniated intervertebral disks and a valid alternative to surgery in many cases.

Paravertebral ozone therapy in sacrolumbar pain produced by disk hernias.
Benítez P.P. and González Y. (Cuba)

The aim of this study was to evaluate the effectiveness of the paravertebral ozone therapy, as a method for low back pain treatment that take place by disk disruption. We study 300 patients, divided at random in five groups of 60 patients each:

  • Group A: Epidural Steroids (control group)
  • Group B: Epidural Ozone
  • Group C: Paravertebral Ozone
  • Group D: Outforaminal Ozone
  • Group E: Intradiscal Ozone

We show in this report that the patients treated with epidural steroids enjoyed only a transitory relief of the pain. However, 95 % of the patients treated with Ozonoterapy had a permanent relief of the pain during the time of evaluation (12 months) and 92 % remained incorporated to their habitual activities.

Oxygen ozone therapy in disk toot compression: Experience in Puebla, Mexico.
Melgarejo A., Arroyo M.L., Moreno V. and Ríos A. (Mexico)

Root compression by an herniated disk is the most common cause of acute or subacute lumbar pain. The use of oxygen ozone therapy has been reported previously as a good alternative treatment. We report our experience in Puebla, Mexico. In this prospective study, we report our initial experience obtained in 32 patients, whom were resistant to conservative treatment for pain caused by herniated or protruded lumbar disks, central or foraminal, demonstrated by clinic, CT and/or MR imaging, electrophysiology and discogram. All patients received during a period of 3 weeks, 8 sessions of a bilateral paraspinal intramuscular injection of 10 mL of oxygen ozone at a concentration of 27 ng/mL at the sensitive level, as well as, 7 - 12 mL of oxygen ozone directly in the disk, this after performing a discogram, to verify the diagnosis and exclude a vascular or arachnoid communication or a major leak, 49 % of cases at the L4/5 level, 39.6 % at the L5/S1 level and 11.3 % at L3/4. All patients entered a lumbar muscular strenghthening program and used an external support. The clinical benefits were immediate in 24 patients (75 %) with total or almost total resolution of symptoms, good results in 6 (18.7 %) and minimum or no benefit in 2 (6.2 %). Recurrence of symptoms presented in 2 patients at 6 months, and in 3 at 10 months, undergoing therefore a second trial, with good results. To this moment, our results are encouraging as to the effectiveness of the use of oxygen ozone therapy in patients resistant to conservative treatment for pain caused by lumbar root nerve compression.

Intradiscal ozone for the treatment of herniated disk. Discolysis
De Lucas-García J.C. and De Lucas-Villarubia J.C. (Spain)

We present our experience in the treatment, using intradiscal ozone, of 100 consecutive cases of cervical and lumbar symptomatic herniated disks (94 patients), treated in our Institution, since November 1999, with a minimum follow-up of 6 months. The patients were evaluated before treatment, at the end, and in the follow-up, using a personal clinical score, based on the one of Lassale (1984) for evaluation of spinal stenosis. Pain was evaluated using an analogous scale for the evaluation of pain (VAS). At the end of the treatment a subjective assessment was carried out. The results demonstrated that at the end of the follow-up we obtained a 83 % of good and excellent clinical results, with just 8 patients needing a surgical procedure (poor result). Pain improved from 3.19 points (severe pain, with no response to medical treatment) to 8.18 points at the end of treatment (moderate pain, does not need medical treatment) and 8.66 points at the last follow-up (mild pain), with 27 patients scoring 10 points (no pain). At the last revision, 85 patients (90 %) were satisfied with the treatment and in similar circumstances, 83 patients (88 %) would repeat it. These data suggest that intradiscal application of ozone is a valid option for treatment of symptomatic herniated disk, with good results and almost absence of complications.

A comparative study between the clinical behavior and the RMI scan resolution in patients undergoing ozone therapy due to acute disk herniation with root compression.
Ríos J.M., Grangeat A., Pérez A. and Croce E. (Argentina)

The treatment of the acute herniation disk can be approached through various methods, some of them being non-surgical like discolisis with oxygen-ozone therapy (O2-O3). The aim of this paper is to analyse the progressive changes occurring in the RMI and the absence of correlation between the former and the clinical improvement of the symptoms in patients undergoing ozone therapy due to acute disk herniation with root compression. Between April 2002 and December 2003, 122 patients with herniation disc pathology underwent oxygen-ozone therapy discolysis. Fourty of them with acute herniation lumbar disk, of whom 28 (70 %) were male and 12 female (30 %). Mean age: 36.89. A hundred percent complained about pain which resulted in: social behaviour changes 30 (75 %), wakefulness 28 (70 %), amusement disturbances 20 (50 %), sexual disturbances 16 (40 %). Other minor disorders were sensory changes like disesthesia, paresthesia, or anaesthesia). Radiology diagnosis was utilised in all cases by means of RMI. Twenty two out of 40 (55 %) had involved L5-S1 space. Heigth (20 %) L4-L5, 6 (15 %) L3-L4, and 4 (10%) had more than one level involved. Test on pain and influence on various aspects were run, which resulted in a pain index inability (IDD). Both RMI and IDD results were later compared. These patients underwent 10 sessions of O2-O3, two of which consisted of 6 mg/dose and 8 of 30 mg/dose. After the treatment, further tests were performed and new IDD results were obtained. These results were later classified as follows: Excellent (35 %); Very Good (50 %); Good (10 %), fairly good (5%) and failure (0%). In fact, ozone-therapy results in an improvement in the disc-nerve root conflict and in the overall clinical picture. Such achievement is independent of the mechanical factor changes, which will take place only later in time (6 - 12 months). It can be clearly observed that patients have improved their symptoms independently of the RMI changes, which occur only six month after ending the treatment. Pain resolution and its concomitant disabilities is the aim of the treatment in patients with acute disc herniation.

Ozonized oil in medicine:

Ozone therapy in the malabsorption syndrome secondary to parasitism by Giardia lambia.
Méndez N.I., Calunga J.L., Menéndez S. and Carballo A. (Cuba)

Intestinal parasitism by Giardia lamblia is a very important health problem. Global statistics report prevalence rates of 2 - 25 %, depending on the countries’ development level and geographic location. In Cuba, the rates are 7 - 15 %. The symptomatology can be an acute phase with diarrhea alternating to constipation, abdominal pain (colic), epigastralgia, flatulence and urticaria. In the chronic phase, diarrhea can be steady, with a severe weight loss, anemia, producing a disordered or inadequate absorption of nutrients from the intestinal tract especially the small intestine, causing the malabsorption syndrome. The efficacy of oral OLEOZON® as antiparasitic therapy for Giardia lamblia has been demonstrated, but in malabsorption syndrome, effectiveness is not so high. Taking into account the beneficial effects of ozone (increase antioxidant defense system, immunemodulator, great germicidal agent, enhance blood oxygenation), the aim of this study is to use oral OLEOZON® in combination with rectal ozone therapy, in patients with malabsorption syndrome. A sample of 186 adult patients, of both sexes, was divided at random in three groups of 62 patients each. Group I, patients treated with metronidazole (1 tablet each 8 h, during 10 days, a week rest and repetition of the treatment) and vitamins (folic acid and intramuscular vitamin B12); group II, patients treated with oral OLEOZON® (20 drops twice per day during 10 days, a week rest and repetition of the treatment) and group III, patients treated with oral OLEOZON® and rectal ozone therapy (ozone concentration of 40 mg/L and a volume of 150 mL, 20 sessions). All the groups received 2 cycles of treatment with an interval of 3 months. Patients were evaluated clinically, by bile drainage and jejunum biopsy, at the beginning and at the end of the treatment. In group III, an improvement of 66 % was achieved; within them, in those early diagnose, 80.6 % healed. In groups I and II the results were 0 and 37 % of efficacy, with significant differences among groups. The combination of rectal ozone therapy and oral OLEOZON® is recommended for the treatment of patients with malabsorption syndrome.

Treatment of the acute ulcerative necrotizing gingivitis with OLEOZON®.
Martínez M., Menéndez S. and Castillo A. (Cuba)

The ulcerative necrotizing gingivitis is a painful and destructive illness of the gingiva, characterized by inflammation, redness, swelling and tendency to bleed. The aim of this study is to evaluate the efficacy of OLEOZON® (sunflower ozonized oil) in the treatment of ulcerative necrotizing gingivitis in an acute phase. A clinical random trial, phase III, simple blind test, was carried out in 48 patients, where OLEOZON® was compared with a control group. The odontologist didn’t know to what group belonged each patient. Experimental group: 24 patients treated with OLEOZON®, topically, 3 times per day, during 7 days. Control group: patients treated, locally, with a solution of sodium perborate, 3 times per day, during 7 days. The healing criteria established were: disappearance of the lesions, pain, bleeding and pseudomembrane. The evaluations were made at the 3rd and 7th days of beginning the treatment. The results demonstrated that at the end of the treatment, in the experimental group 75 % of patients cured in comparison with the control group (29.2 %), with significant differences (p = 0.01) between both groups. Also, in the experimental group the disappearance of symptoms was achieved more rapidly in comparison with control group. No side effects were observed. It was demonstrated that OLEOZON® is more effective in comparison with sodium perborate in the treatment of patients with acute ulcerative necrotizing gingivitis, being an appropriate therapeutic option for this disease.

Use of OLEOZON® in vaginal monilia treatment.
Elías-Calles B., Savigne M.E. and Sayoux C. (Cuba)

Monilia or Candida albicans mycosis is a yeast vaginal infection. OLEOZON® (ozonized sunflower oil) has a broad anti microbial spectrum, registered as antifungal product in Cuba. In vitro studies, it has shown a great efficacy against yeasts. Taking into account its germicidal properties, a group of 483 women (31 - 45 years old), suffering of vaginal monilia, confirmed by clinical symptoms and microbiological tests, were treated with OLEOZON®. A cycle of daily vaginal embrocation, during 10 days, was employed. If the treatment was not effective, a second cycle of treatment was applied. The results demonstrated that 81.2 % of the patients showed no evidence of clinical symptoms and 82.3 % of cases showed negative microbiological tests after a first cycle treatment. The use of a second cycle, showed no evidence of clinical symptoms in 84.3 % of cases and negative microbiological tests in 87.2 % of patients.

Generalization of the use of OLEOZON® in the treatment of tinea pedis. Preliminary study
Falcón L., Daniel R.D. and Menéndez S. (Cuba)

Tinea pedis is a frequently occurring disease which produces different tissue lesions as desquamation, maceration and pruritus. This clinical syndrome is often resistant to treatment and frequently relapses occur. Ozonized sunflower oil, OLEOZON®, has a remarkable germicidal action. In a preceding paper, it has been demonstrated the efficacy of OLEOZON® in patients with tinea pedis, in a phase III clinical trial, using Nizoral as control group. The aim of this study was to generalize the use of OLEOZON® in patients with tinea pedis. Four hundred and forty three, in- and out- patients, from "Dr Carlos J. Finlay" Military Hospital were treated topically (once a day) with OLEOZON®, during 6 weeks. The results demonstrated that 381 patients (86 %) were healed and the most effective results were found in scaled and macerated clinical forms. Slight side effects were only seen in three patients.

OLEOZON®: An effective treatment in the management of pyodermitis.
Daniel R.D., Falc&oacuten; L., Moya S., Garbayo E. and Menéndez S. (Cuba)

Pyoderma is an acute, inflammatory, purulent disease. Taking into account the germicidal power of ozonized sunflower oil (OLEOZON®), we decided to study the efficacy of it in the treatment of pyoderma and to compare it with Neobatin, as conventional drug. A sample of 1098 out-p atients, with clinical and bacteriological diagnosis of pyoderma, was included in this study. The sample was divided at random into two groups: OLEOZON® group and control group, using Neobatin ointment treatment. It was indicated, in both groups, the use of physiological saline compress, twenty minutes before topical application of the specific treatment, in order to remove any crust. Treatments were applied twice daily, during ten days. The results indicated that 90.1 % of the OLEOZON® treated patients and 81.6 % of the Neobatin ointment treated patients were negative in the bacteriological test, with statistical significant differences between groups. We concluded that OLEOZON® is an effective treatment in the management of pyoderma.

Application of OLEOZON® in vulvovaginitis.
Rubio R., El&iacute-Calles B., Favier M., Castellanos G. and Lescaille E. (Cuba)

The aim of this study is to evaluate the efficacy of OLEOZON® in patients with vulvovaginitis. Three hundred fifty women were treated with vaginal embrocations of OLEOZON®, once a day, during 10 days. Microbiological vaginal exams were performed at the beginning and at the end of the treatment. The women age group more affected was 25 - 34 years, and all were workers. Candida albicans was the most frequent microorganism present in this study. Leucorrhea was the symptom more frequent and it disappeared since the 3rd day of treatment, as well as pruritus. The evaluation criteria were disappearance of signs and simptoms and a negative microbiological result. At the end of the treatment, 95 % of patients cured. OLEOZON® can be a good medication for the treatment of vulvovaginitis.

Evaluation of therapeutic properties of OLEOZON® in the treatment of women with Human Papilloma Virus (HPV) uteri cervix infection.
Ríos M.A., Aguilar O., Torres A., Hernández M., Menéndez S. and Amigó M. (Cuba)

HPV is associated with cervical cancer and preceding lesions. Cervical cancer is the second most common malignant tumor in the world, although its tendency is to occupy a first place in some geographic zones, such as Central and South America and the Caribbean region. In spite of several studies made worldwide to establish effective therapeutics against HPV infection, there isn't consensus about this. Now there are two different treatments for HPV infections. The first is based in lesion destruction by mean of chemicals or surgical methods, but they can't eliminate viral infection. The second one is based in viral elimination with antiviral agents. The ozonized sunflower oil (OLEOZON®) has shown antiviral activity in the treatment of viral infections such as Herpes virus. Our main aim in this study was to evaluate the therapeutic efficacy of this product in HPV infected women. To develop this study we selected 12 women with NIC I, associated with HPV and condiloma acuminate lesions. OLEOZON® was applied daily, in the uteri cervix of each patient, during 20 days. Cytological, histological (in some cases) and colposcopic examination were made to each patient before and after the treatment. Two of the twelve left the treatment. From the rest, eight (80 %) women showed total clinical and cytological regression of lesions. Viral persistence was observed in two (20 %) patients. In some cases, total elimination of vaginal secretion, was observed after the treatment. Side effects due to the OLEOZON® were not observed in any case.

The ozone therapy in vascular diseases and other applications:

Ozone therapy treatments in vascular pathology in the region of Murcia, Spain.
Batanero J., Abadia F., Ruíz J.A. and Felices J.M. (Spain)

Since April 2003 we have treated 106 patients of different pathologies in relation with cardiovascular diseases, mainly vascular ulcers, (diabetic lesions ischemic disease, venous ulcers) as well as vasoespastic disorders and some cases of telangiectasies in both legs and spiders in chest and face. In order to obtain best results we make transfusions of ozonized blood and local application of ozone and ozonized water in some ulcers. In small numbers of patients we have used rectal applications, using the Humazon Promedic device. Recently, we have treated also pain due to muscular contractions, bursitis and paravertebral pathologyes with good results. We will present photos and comparative results in our final communication.

Platelet inhibition in patients with vascular diseases submitted to ozone therapy treatment.
Díaz A., García M., Piña and Menéndez S. (Cuba)

The aim of this study is to verified whether there is or not inhibition of platelet aggregation after ozone treatment, in patients diagnosed with an ischemic or neuroinfectious diabetic foot, chronic venous insufficiency and subacute arterial ischaemia. Ozone was applied daily, by rectal insufflation, at a concentration of 50 mg/L and 200 mL, in a cycle of 20 sessions. Platelet aggregation was measured at the beginning and at the end of ozone treatment. Platelet aggregation in platelet rich plasma (200 µL) was induced by adding increasing concentrations of adenosine diphosphate, collagen and ephinephrine. We observed that at the end of the treatment, platelet aggregation percentage was reduced to a mean of 14 to 28 %, in comparison with the initial values.

Efficiency of ozone therapy in patients with hypertonic angioencephalopathy.
Kontorschikova C.N., Litvinova N.Y. and Usmanova A.I. (Russia)

We chose 3 groups of patients with Hypertonic Angioencephalopathy (HAEP) in the age of 24 - 56: first group got 10 velotrainings as average per course of treatment (30 - minutes daily trainings with 60 - 75 % threshold loads), second group received intravenous injection of ozonated physiological solution (5 procedures every other day with ozone’s concentration 300 µg/L in 200 mL volume) and third group with velotrainings and ozone therapy. All groups received hypotensive medications. Lipid peroxidation (LP) and antioxidant activity (AOA) control tests were done before and after the treatment, which included chemiluminogramme assessment (Imax/S, Imax/TL, S/TL, tg 2a/TL), analysis of dien and trien conjugates(DC, TC) and of Shiff bases (SB). There was obtained Imax/S decrease (from 0.26 to 0.14, p = 0.03) and DK and TK increasing tendency in the first group. LP initial products - DK and TK, LP final products - SB, Imax/TL, S/TL had a tendency to be decreased in the second group. There was observed S/TL decrease (from 35.74 to 22.31, p = 0.04), TK, SB, Imax/TL decreasing tendency and Imax/S increasing tendency in the third group, thus testifying the increase in AOA. Taking into consideration sedentary lifestyle of the patients and short duration of trainings we propose physical loads to be a stress factor with a decrease in AOA. Ozone and velotrainings combination promotes the stress adaptation.

Ozone therapy in treatment of discirculatory encephalopathy. Psychological aspects.
Nelaeva I.A. (Russia)

The cerebrovascular pathology and its dangerous sequela - the cerebral insult is the most highly social and medical problem not only in Russia but all over the world. This disease takes the second place (21.4 %) among the general mortality rate in Russia. The discirculatory encephalopathy is the slowly progressing insufficiency of the brain circulation. The nootropil, vascular drugs, antiaggregates, vitamins, antioxidants and antidepressants are used in the treatment of discirculatory encephalopathy traditionally. The main index for the use of ozone therapy treatment in patients with discirculatory encephalopathy is the insufficient oxygen supply of the brain and its assimilation. All the patients were undergone the clinical neurologic examination in addition to ophthalmoscopy, craniography and experimental psychological check up. The 24 patients with the discirculatory encephalopathy (I and II stages) have been examined and treated.The intravenous drop infusions of the ozone physiological solution were carried out daily. The cycle of ozone therapy treatment was 8 - 10 procedures. The analysis of the clinical evolution of the experimental psychological investigation, at the end of the ozone treatment, showed the decrease of the main complaints in 17 patients with discirculatory encephalopathy (70.8 %). So, our previous data demosntrated the good effects achieved using ozone therapy in the treatment of discirculatory encephalopathy.

Use of ozone therapy in the stroke.
Castillo P. and Salas T. (Cuba)

The cerebrovascular disease is one of the main problems in medical practice, because of the high tendency to produce sequels in those who survive, limiting their physical capacities. The purpose of this study was to evaluate the effectiveness of ozone therapy in the ischemic cerebrovascular disease, in acute phase. One hundred patients, with this diagnosis, that attended the Neurological Institute during a period of one year, were evaluated. Ozone by rectal insufflation was daily administered (at an ozone concentration of 40 mg/L and 200 mL), for a total of 15 sessions. These patients were evaluated by clinical examination, blood test and their validity through the multidimensional evaluation method, at the beginning and at the end of the treatment. Antiaggregant medication was associated. The clinical stage improved in 80 % of patients treated with ozone therapy, with a satisfactory increase of their quality of life.

Is therapeutic ozone genotoxic?
Díaz S. (Cuba)

Ozone is a potent oxidant toxic agent as environmental pollutant to human airways. However, it is applied as chemotherapeutic agent under controlled conditions and proper concentrations to diverse disturbances expressed through deficit of antioxidant defences, due to its proved effect to stimulate them. It has also been confirmed that ozone is mutagenic in microorganisms and genotoxic in animals and human cells in vitro. We studied the genotoxic activity of therapeutic ozone in animal models, human cells in vitro and in patients receiving this therapy. Two levels of genetic damage were analysed, cytogenetic damage by chromosomal aberrations assay and primary lesions to DNA by the Comet assay. The results showed that therapeutic ozone is not clastogenic in patients, but induces DNA single strand breaks in exposed cells probably through its reactive intermediaries as hydrogen peroxide. The recovery of the cell damage was followed after the treatments confirming that DNA damage declines after the end of exposure.

Social economic impacts of 11 years in the application of ozone therapy.
Mapolón Y., Recio E., Palma M., Rodríguez M. and Harrys C. (Cuba)

A descriptive study with patients treated with ozone therapy, from July 1992 till December 2003, was carried out at the "Orfilio Peláez Molina" Provincial Center of Retinitis Pigmentosa, of Camagüey Province. The whole sample treated with ozone was 168 310 patients (children and adults). The ozone concentrations used were between 30 and 40 mg/L. The ozone administration ways used were: rectal, autohemotherapy, intramuscular, subconjunctival and local treatment using gas bag and ozonized oil. Patients received more than five cycle of ozone treatment. The medical fields were ozone was more applied were: opthalmology (Retinitis Pigmentosa with more than 12 000 patients treated, glaucoma, maculopathy, etc.), neurology, orthopedics, angiology, rheumatology, geriatrics, among them. Rectal administration was the way of application more used. In general, the clinical results were very good, taking into account the high percent of patients that showed a recovery in signs and symptoms of their illness, without side effects. According to an economical point of view, ozone therapy was cheaper than the conventional treatments with antibiotics, analgesics, anti-inflammatory drugs, etc. Savings of 138 431.95 pesos were achieved. All these results are a proof of the effectiveness and advantages of this therapy.

Randomized study comparing the safety and efficacy of ozonized oil (OLEOZON®) and Ornidazol (Tiberal®) in the treatment of giardiasis.
González M.A., Menéndez S., Palomino A., Amoroto M., Fernández M. and Calunga J.L. (Cuba)

Giardiasis is one of the most common pathogenic intestinal protozoal infections worldwide. The adverse effects and treatment failures to some of the currently recommended drugs for Giardia lamblia infection have given rise to the need for alternative antigiardiasis agents. Taking into account the remarkable germicidal action of the sunflower ozonized oil (OLEOZON®), stability and low cost, the aim of this paper is to determine, in an open, randomized parallel group study, the safety and efficacy of this ozonized oil in comparison with Ornidazol (Tiberal®) for the treatment of giardiasis. Two hundred and twenty four outpatients of either sex (age range: 17 - 71 years) and race, with a parasitologic diagnosis of giardiasis, were randomised to two groups: OLEOZON® group - 112 patients received 20 drops of OLEOZON®, twice per day, during two cycles of 10 days each, with a rest of one week between cycles of treatment. Tiberal® group - 112 patients received a tablet of 500 mg of Tiberal®, twice per day, during 5 days. Patients’ written acceptance to participate in the study was taken into account. Both groups were homogenous in age, sex, race and presence of previous episodes of giardiasis (almost 60 % of patients presented relapses and have been treated previously with imidazoles). The main response variable was the absence of cysts or trophozoites of Giardia lamblia in duodenal fluid. Also the presence of symptoms and side effects were taken into account. At the end of the treatments, equal efficacy for both medications (OLEOZON®: 64.3 %, Tiberal®: 65 %) was obtained. Symptom behaviour was the same for both groups (~50 % were symptom free). Upper abdominal pain and diarrhea prevailed. In the Oleozon group, 2 % presented vomits, maybe because of the taste and odour of the product and its way of administration (drops). It was demonstrated that OLEOZON® can be an alternative medication in the treatment of giardiasis, taking into account that most of these patients develop resistance to imidazole products.

Current trends on biomedical applications of ozone and ozonized vegetable oils. Preliminary patent study.
Herrera V., García B. and Ledea O. (Cuba)

The outstanding results obtained in biomedical ozone applications and ozonized vegetable oils has produced an economic growing interest as it is demonstrated by the numerous current patent requests. The aim of this work was the identification of the world current trends on medical ozone applications and on medical uses of ozonized vegetable oils. All the Internet free access databases of patent were peer reviewed, employing the automatic system of patent guard (SiVigPat, v.3). The study included the main ozone medical applications, taking into consideration the ozone administration way (intravenous, intra-arterial, intramuscular, subcutaneous, intra-articular, colorectal, vaginal, and cutaneous, among others), the medical specialties (Oncology, Ophthalmology, Cardiology, Dermatology, Orthopedics, Immunology, Pediatrics, Geriatrics, etc.) and the types of medicine (Preventive, Sport, Aesthetic, etc.). Also, the medical uses of ozonized vegetable oils, the type of vegetable oil employed and the different vegetable oil ozonation processess were evaluated. Furthermore, the information of the main personalities, companies and countries related to the biomedical applications of ozone or ozonized vegetable oils was obtained.

© Copyright 2004. Ferrer L., Góngora, Santos D., Pérez and Menéndez S.

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